Figure 11.
DKO lymph nodes are anti‐inflammatory. The cervical lymph nodes draining the casted artery were stained for Tregs (CD3+CD4+ Foxp3+) and analyzed by flow cytometry. (A) Representative plots of CD4 and Foxp3 in the CD3+ gate (left) and the percentage of double positives for 3 Apoe−/− and 4 DKO (right). (B) qRT‐PCR of lymph nodes for transcriptional regulators (top) and polarizing cytokines (bottom). Data are presented as mean±SEM (3 Apoe−/− and 4 DKO) lymph nodes. Apoe−/− white bars, DKO black bars. Significance was determined by independent sample t test, *P<0.05. Apo indicates apolipoprotein; DKO, double knockout; Foxp3, forkhead box protein 3; qRT‐PCR, quantitative real‐time polymerase chain reaction; Tregs, regulatory T cells.