Figure 3.

EXT2 knockdown does not lead to further increase in NOS3 pathway. The effect of EXT2 knockdown on RNA expression of EXT2 (A), EXT2 (B), NOS3 (C), NRF2 (D), KLF2 (E) in HMVEC under laminar flow analyzed by RT‐PCR. EXT1 was silenced using siRNA against EXT2 as described. A scrambled siRNA was used as control. Flow induces a significant increase in NOS3, NRF2, and KLF2, which was not further increased by downregulation of EXT2. Data are from 4 independent experiments and presented as mean±SD. All silencing experiments were performed under laminar flow condition. Differences were analyzed using a nonparametric statistical test (Mann–Whitney). Differences were analyzed using Student t test. *P<0.05; ***P<0.005. EXT indicates exostosin; HMVEC, human microvascular endothelial cell; KLF2, kruppel like factor 2; NOS, nitric oxide synthesis; NRF‐2, nuclear factor erythroid 2?related factor; RT‐PCR, reverse‐transcription polymerase chain reaction.