Table 2.
Effects of dietary supplementation with FF, FF/AqSF-P, and FF/BY on hepatic weight, hepatic index, and liver function in ND-and HCBD-fed mice
| Groups | Dose (%/w/w) | Hepatic weight (g) | Hepatic index | Serum ALT activity (U/L) |
|---|---|---|---|---|
| ND-fed mice | ||||
| ND | n/a | 1.71±0.07 | 6.01±0.16 | 40.80±7.10 |
| FF | 0.1 | 3.00±0.11** | 11.46±0.19** | 121.70±14.14** |
| FF/BY | 0.1/0.025 | 3.18±0.07** | 11.81±0.17** | 55.60±5.83†† |
| FF/AqSF-P | 0.1/0.3 | 3.19±0.07** | 11.87±0.17** | 134.60±13.96** |
| 0.1/1 | 3.07±0.06** | 11.90±0.25** | 149.14±23.77** | |
| 0.1/3 | 3.00±0.14** | 11.40±0.28** | 127.30±12.15** | |
| 0.1/9 | 2.97±0.08** | 11.67±0.23** | 88.30±5.95** | |
| HCBD-fed mice | ||||
| ND | n/a | 1.74±0.13 | 6.09±0.40 | 69.40±1.28 |
| HCBD | n/a | 2.10±0.07* | 7.33±0.11** | 81.00±1.05** |
| FF | 0.03 | 3.51±0.08## | 11.61±0.21## | 122.80±7.28## |
| FF/BY | 0.03/0.025 | 3.49±0.12## | 11.69±0.21## | 71.50±4.51†† |
| FF/AqSF-P | 0.03/3 | 3.25±0.12## | 11.09±0.20## | 108.70±8.15## |
| 0.03/9 | 3.52±0.10## | 11.99±0.18## | 100.00±4.44##,† | |
| HCBD-fed mice | ||||
| ND | n/a | 1.75±0.04 | 6.32±0.07 | 60.00±3.38 |
| HCBD | n/a | 2.07±0.03** | 7.38±0.09** | 86.90±7.48** |
| FF | 0.1 | 3.69±0.21## | 14.48±0.48## | 191.13±35.94## |
| FF/BY | 0.1/0.025 | 3.79±0.12## | 14.44±0.24## | 150.78±21.79# |
| FF/AqSF-P | 0.1/1 | 3.77±0.06## | 14.31±0.18## | 296.71±63.52## |
| 0.1/3 | 4.05±0.12## | 14.72±0.31## | 288.00±38.37## | |
| 0.1/9 | 3.46±0.12## | 14.10±0.32## | 378.56±57.42## | |
Notes: Mice were fed with ND or HCBD to establish a model of hypercholesterolemia. FF or the combination of FF and AqSF-P or BY at the indicated doses were supplemented with either ND or HCBD. The concentrations of AqFS-P were estimated on the basis of crude herbal material. Ten days after the dietary supplementation, hepatic weight/index and serum ALT activity were measured. Hepatic index was calculated from the following equation: hepatic weight/body weight ×100. Values given are the means ± SEM, with n=10.
P<0.05
P<0.01 vs ND
P<0.05
P<0.01 vs HCBD
P<0.05
P<0.01 vs FF alone. Statistically significant differences were determined using a one-way ANOVA, followed by Dunnett’s multiple-comparisons test.
Abbreviations: ND, normal diet; HCBD, high cholesterol/bile salt (0.5%/0.15%, w/w) diet; FF, fenofibrate; AqSF-P, aqueous extract of Schisandrae Fructus pulp; BY, bicyclol; ALT, alanine aminotransferase; SEM, standard error of mean; ANOVA, analysis of variance; vs, versus; w/w, weight/weight; n/a, not applicable.