Table 3.
Effects of dietary supplementation with FF, FF/AqSF-P, and FF/BY on the organ indexes in ND- and HCBD-fed mice
| Groups | Dose (%/w/w) | Organ weight/body weight ×100
|
Organ weight/body weight – liver weight ×100
|
||||
|---|---|---|---|---|---|---|---|
| Fat | Kidney | Testis | Fat | Kidney | Testis | ||
| ND-fed mice | |||||||
| ND | n/a | 1.23±0.08 | 1.47±0.04 | 0.63±0.02 | 1.32±0.08 | 1.57±0.05 | 0.67±0.02 |
| FF | 0.1 | 0.71±0.04** | 1.57±0.04 | 0.68±0.03 | 0.80±0.05** | 1.77±0.04** | 0.76±0.04* |
| FF/BY | 0.1/0.025 | 0.70±0.03** | 1.48±0.04 | 0.65±0.02 | 0.79±0.03** | 1.68±0.05 | 0.74±0.03 |
| FF/AqSF-P | 0.1/0.3 | 0.69±0.04** | 1.59±0.04 | 0.66±0.03 | 0.78±0.05** | 1.80±0.05** | 0.75±0.03* |
| 0.1/1 | 0.62±0.05** | 1.57±0.03 | 0.74±0.02** | 0.70±0.06** | 1.78±0.04** | 0.84±0.02** | |
| 0.1/3 | 0.66±0.05** | 1.57±0.04 | 0.68±0.03 | 0.75±0.06** | 1.77±0.05** | 0.77±0.03* | |
| 0.1/9 | 0.75±0.06** | 1.52±0.03 | 0.76±0.03** | 0.85±0.06** | 1.72±0.04* | 0.86±0.03** | |
| HCBD-fed mice | |||||||
| ND | n/a | 1.17±0.07 | 1.49±0.03 | 0.47±0.05 | 1.25±0.08 | 1.59±0.03 | 0.50±0.05 |
| HCBD | n/a | 0.87±0.05** | 1.54±0.03 | 0.54±0.03 | 0.94±0.06** | 1.66±0.03 | 0.58±0.03 |
| FF | 0.03 | 0.59±0.03## | 1.47±0.03 | 0.47±0.02 | 0.67±0.04## | 1.67±0.04 | 0.53±0.02 |
| FF/BY | 0.03/0.025 | 0.54±0.02## | 1.51±0.04 | 0.46±0.02# | 0.62±0.02## | 1.71±0.04 | 0.52±0.02 |
| FF/AqSF-P | 0.03/3 | 0.61±0.02## | 1.58±0.03† | 0.48±0.02 | 0.69±0.03## | 1.78±0.04#,† | 0.54±0.02 |
| 0.03/9 | 0.62±0.03## | 1.60±0.05† | 0.48±0.01 | 0.70±0.04## | 1.82±0.06#,† | 0.54±0.01 | |
| HCBD-fed mice | |||||||
| ND | n/a | 1.03±0.07 | 1.63±0.04 | 0.61±0.02 | 1.10±0.07 | 1.75±0.05 | 0.65±0.02 |
| HCBD | n/a | 0.90±0.05 | 1.62±0.04 | 0.58±0.02 | 0.97±0.06 | 1.75±0.04 | 0.62±0.03 |
| FF | 0.1 | 0.50±0.04## | 1.64±0.07 | 0.64±0.03 | 0.58±0.05## | 1.92±0.07# | 0.75±0.03## |
| FF/BY | 0.1/0.025 | 0.53±0.02## | 1.56±0.05 | 0.58±0.02 | 0.62±0.02## | 1.82±0.06 | 0.68±0.03 |
| FF/AqSF-P | 0.1/1 | 0.57±0.03## | 1.70±0.04 | 0.65±0.02 | 0.67±0.03## | 1.98±0.05## | 0.75±0.03## |
| 0.1/3 | 0.57±0.03## | 1.67±0.04 | 0.58±0.02 | 0.67±0.04## | 1.95±0.05## | 0.68±0.03 | |
| 0.1/9 | 0.41±0.03## | 1.57±0.04 | 0.61±0.04 | 0.48±0.03## | 1.83±0.04 | 0.71±0.05 | |
Notes: Mice were fed with ND or HCBD to establish a model of hypercholesterolemia. FF or the combination of FF and AqSF-P or BY at the indicated doses were supplemented with either ND or HCBD. The concentrations of AqFS-P were estimated on the basis of crude herbal material. Ten days after the dietary supplementation, organ indexes were estimated. Fatty index was obtained from the epididymal fat pad. Values given are the means ± SEM, with n=10.
*P<0.05
**P<0.01 vs ND
P<0.05
P<0.01 vs HCBD
P<0.05 vs FF alone. Statistically significant differences were determined using a one-way ANOVA, followed by Dunnett’s multiple-comparisons test.
Abbreviations: ND, normal diet; HCBD, high cholesterol/bile salt (0.5%/0.15%, w/w) diet; FF, fenofibrate; AqSF-P, aqueous extract of Schisandrae Fructus pulp; BY, bicyclol; SEM, standard error of mean; ANOVA, analysis of variance; w/w, weight/weight; vs, versus; n/a, not applicable.