Figure 1.

Rarefaction analysis of D. pulchra microbial community samples with OTUs defined at a 16S rRNA V4 region sequence identity of 97%. Bleached (B) and healthy (H) samples from Bare Island (BI) and Long Bay (LB) are shown. The thick dashed line, which essentially overlaps with the y-axis, shows the sequencing depth of a previous study based on 16S rRNA gene clone libraries (Fernandes et al., 2011). The thin dashed line shows the minimum number of sequences for any sample analyzed here and to which level all samples were subsampled for subsequent community comparison.