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. 2015 Feb 3;6:6146. doi: 10.1038/ncomms7146

Table 1. GLMM of the influence of parental age on egg hatching success.

Term d.f. Effect s.e. Wald stat (χ2) P value
Day 1 0.01144 0.005035 5.16 0.023
Day2 1 −0.00009522 0.000036212 6.91 0.009
Day3 1 0.0000001941 8.1796E−08 5.63 0.018
Clutch number 1 0.2659 0.04786 30.88 <0.001
Clutch number2 1 −0.09423 0.027588 11.67 <0.001
Within clutch order 1 0.1597 0.06327 6.37 0.012
Within clutch order2 1 −0.2615 0.06610 15.65 <0.001
Maternal age 1 4.083 0.6218 43.13 <0.001
Maternal age2 1 −0.2845 0.04296 43.87 <0.001
Paternal age 1 1.587 0.5048 9.88 0.002
Paternal age2 1 −0.1089 0.03483 9.78 0.002

d.f., degree of freedom; GLMM, Generalized Linear Mixed Model; s.e., standard error.

The aim of the analysis was to examine the potential influence of parental aging on the hatching success of their eggs. A binary variable was used which indicated whether eggs that had been incubated subsequently hatched. A logistic GLMM was implemented using a logit link function; constant=−20.06. In total, the analysis includes assessments of 58,977 eggs laid by a total of 2,580 females and fertilized by a total of 1,161 different males. Parental identity and year of breeding were controlled as random effects. Variables derived from parental age and clutch sequences data were loge transformed to improve model fit.

Numbers in superscript indicate that the terms have been fitted as a second/third order polynomial.