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. 2015 Feb 19;15:74. doi: 10.1186/s12879-015-0799-x

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© Ferraz et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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In vitro analysis of effector (A – E) and apoptotic-effector (F - J) CD8 ⁺ T-lymphocyte frequencies. (A and F): comparison among the percentages of Leishmania braziliensis antigen (LbAg)-stimulated cells from HS - healthy subjects (n = 8); PBT – patients before treatment (n = 8); PDT - patients during treatment (n = 12); and PAT - patients after treatment (n = 6). Statistical analyses were performed by Mann Whitney Test and the bars represent the mean ± standard error. (B, C, D, E, G, H, I, J): comparison between antigen-stimulated (Lb-Ag) and nonstimulated cells (BG – background) from HS; PBT; PDT and PAT, respectively. Solid lines connect the results for the same individual. Statistical analyses were performed by paired, nonparametric Wilcoxon test. Results were considered significant with P < 0.05. ^*(P < 0.05) **(P < 0.01) ***(P < 0.001).