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. 2006 Feb 12;82(9):339–352. doi: 10.2183/pjab.82.339

Fig. 7.

Fig. 7.

Nrf2/MafK binds and activates GPE1, a strong enhancer element of GST-P gene.28)
  1. EMSA was performed with Nrf2/MafK and MafB proteins and GPE1, mGPE1, and mmGPE1. The nucleotide sequences of the probes are shown. Mutated positions are indicated by underlines.
  2. DNase I footprinting analysis of Nrf2/MafK with GPE1 probe. (+) and (−) indicate the probe with or without Nrf2/MafK proteins. Guanine and adenine residues of the same probe were cleaved by Maxam and Gilbert methods (M). Arrows indicate TRE-like sequences.
  3. Reporter transfection analysis of wild- and mutated-GPE1 in F9 cells. Indicated reporter plasmid or promoter-less luciferase plasmid (Vector, pGVB2) was cotransfected with expression plasmid of Nrf2 (black), c-Jun (gray), or without expression vector (open). Because of the abundance of MafK in F9 cells, MafK expressing plasmid was not used here, to avoid sequelching.