Figure 5. ADAM17 regulates the surface levels of CXCR2 in vivo. (A) Control and conditional ADAM17-knockout mice were administered LPS i.p. (5 mg/kg). CXCR2 surface levels on peripheral blood neutrophils from both groups of mice were determined by flow cytometry, before and after (4 h) LPS administration. Isotype-negative control antibody staining is indicated by a dotted line, the x-axis = Log 10 fluorescence, and the y-axis = cell number. (B) Some cells were subjected to CXCR2 recycling, as described in Fig. 4. The mean fluorescence intensities of CXCR2 staining for control and ADAM17-null neutrophils are displayed as box-whisker plots of 5 independent experiments, which depict the smallest value, lower quartile, median, upper quartile, and largest value. (C) For mice that were administered LPS, CXCR2 mean fluorescence intensity data are presented as a percent change relative to control neutrophils before recycling. The bar graph shows mean ± sd of 5 mice/group (control or conditional ADAM17-knockout mice). Statistical significance is indicated as *P < 0.05, and ***P < 0.001.