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. 2014 Dec 3;89(4):2220–2232. doi: 10.1128/JVI.03385-14

FIG 2.

FIG 2

HCV production in Huh-7.5.1-8 cells grown under serum-free conditions. Huh-7.5.1-8 cells were infected with HCV JFH-1 and then cultured in serum-free medium. (A) The cells were harvested at the indicated time points. Each cell lysate (15 μg of protein) was subjected to immunoblotting for core, NS3, and GAPDH proteins. (B) Culture supernatants were harvested at the indicated time points. The amount of secreted viral particles in each supernatant was determined by measuring the amount of core protein by ELISA. The results from one of two independent experiments with similar results are shown.