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The anti-HCV effect of YM-53601 is significantly reversed by LDL. Huh-7.5.1-8 cells were infected with HCV JFH-1 and then treated with 1 μM YM-53601 or DMSO in serum-free medium that contained 0 to 100 μg/ml of LDL for 5 days. An equal portion of each cell lysate was subjected to immunoblotting for core, NS3, and GAPDH proteins. The results from one representative experiment performed in triplicates are shown. Similar results were obtained in two independent experiments.
