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Western blot analysis of transgenic RK13 cells expressing pUL31 NLS and NES mutant proteins. RK13 cells stably expressing native pUL31 or pUL31 mutant proteins were lysed, and proteins were separated by SDS–10% polyacrylamide gel electrophoresis. After transfer onto a nitrocellulose membrane, the blot was incubated with polyclonal monospecific rabbit anti-pUL31 serum. A control blot was incubated with a murine anti-α-tubulin monoclonal antibody.
