FIG 8.

Localization of mutated pUL31 and wild-type pUL34 during PrV infection. To investigate the interaction of mutated pUL31 with pUL34 during virus infection, the corresponding cell lines were incubated with PrV-ΔUL31 (A) or PrV-Ka (B) under plaque assay conditions and fixed 2 days p.i. Localization of pUL31 and pUL34 was analyzed by confocal laser scanning microscopy after an indirect immunofluorescence assay with monospecific rabbit anti-pUL31 (red) and murine anti-pUL34 (green) sera. DAPI (blue) was included to visualize nuclei. Bars, 10 μm.