TABLE 1.
Primers used for mutagenesis of PrV pUL31
| Name | Sequence (5′ to 3′)a | Location in PrV-Ka (nt)b |
|---|---|---|
| UL31for | CTGAATTCACACGCTCGGCAGCTATGTTTG | 29581–29573 |
| UL31rev | CTGAATTCTTCGCGGCGCTCACGG | 29771–29766 |
| UL31-ΔNLSfor | CACAGAATTCATGGATCGCTACGCGCCCTA | 29506–29490 |
| UL31-ΔNESrev | CTGAATTCCGCTCAGTCCCGCATCTTGCAATAAATG | 28868–28847 |
| UL31-NLSPM1-1 | GCTATGTTTGAGCGAGCGGCGCTCCTGCGGCGCAAG | 29584–29549 |
| UL31-NLSPM1-2 | CTTGCGCCGCAGGAGCGCCGCTCGCTCAAACATAGC | 29549–29584 |
| UL31-NLSPM2-1 | AAGTCGTCGGCCGCGGCGGCCAAGACGCTGACGCGC | 29551–29516 |
| UL31-NLSPM2-2 | GCGCGTCAGCGTCTTGGCCGCCGCGGCCGACGACTT | 29516–29551 |
| UL31-NESPM1-1 | GCTTCGACGGGGAGGCGCTGGCGGAGTACAAAAGATTG | 28842–28805 |
| UL31-NESPM2-2 | CAATCTTTTGTACTCCGCCAGCGCCTCCCCGTCGAAGC | 28805–28842 |
a
Restriction sites introduced for convenient cloning are underlined, start and stop codons are shown in bold, and mismatches for site-directed mutagenesis in italics.
b
Positions correspond to the sequence with GenBank accession number JQ809328. nt, nucleotides.