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Growth curves of H7 mutant VACVs. BSC-1 (A) and BSC-H7 (B) cells in 12-well plates were infected with the indicated viruses at a multiplicity of infection (MOI) of 5 PFU/cell. Viral titers at 0, 24, and 48 h postinfection (hpi) were determined by plaque assay on BSC-H7 cells. The mutants were studied in groups in three separate experiments, each with a WT control.
