TABLE 2.
Summary of phenotypes of H7 mutant VACVs
| H7 mutation(s)a | Plaque sizeb | One-step growthc |
|---|---|---|
| WT | ++++ | ++++ |
| K108E/R109E/K112E | − | − |
| K108E | − | + |
| R109E | +++ | +++ |
| K112E | + | ++ |
| K102E | ++++ | ND |
| K113E | ++++ | ++++ |
| R115E | ++++ | ND |
| F32A | ++++ | ND |
| K33E | ++++ | ND |
| C89S | ++++ | ++++ |
| Δ(119-146) | − | − |
| R121E | ++++ | ++++ |
| K128E | − | − |
| K130E | ++++ | ++++ |
| K135E | +++ | +++ |
| D138K | ++++ | ++++ |
| E140K | ++++ | ++++ |
| K143E | − | − |
| D144K | ++++ | ++++ |
a
Underlining indicates H7 mutants with impaired viral replication.
b
−, no plaques; ++++, WT plaque size.
c
−, no growth after 48 hpi; ++++, growth rate of WT virus; ND, not determined.