FIG 6.

Transient transfection of leader RNA from wt or laboratory-attenuated RABV can induce activation of DCs in a dose-dependent manner. (A) Schematic representation of enzymatic synthesis of rabies leader ssRNA. (B) In silico calculations of minimum free energy secondary structures of CVS-B2c and DRV leader RNA. The structures are colored according to base-pairing probability, ranging from 0 (least likely; red) to 1 (most likely; violet). The 5′ end is indicated by the black arrow. (C) Indicated concentration of enzymatically synthesized leader RNA was used to transfect DCs using Lipofectamine 2000 (lipofectamine con). The number of CD86⁺, CD80⁺, and MHC-II⁺ DCs was measured using flow cytometry.