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Cloning strategy for the generation of SHIVs expressing early HIV-1 env sequences. Two unique restriction sites, ClaI and AgeI, in the SHIV-KB9 infectious clone were utilized. Fragments containing KB9 sequence from the ClaI site to the env ATG start codons and early clade C env sequences with AgeI site designed at the corresponding 3′ end were synthesized and replaced the corresponding regions in the SHIV-KB9-AC.
