TABLE 1.

Cloning and replication of 293T-derived SHIVs in human and rhesus PBMC^a

HIV-1 Env	Cloning in SHIV-KB9	Infectivity titer (TCID50/ml)
		293T cells	Human PBMC	Rhesus PBMC
072c	Yes	1.25 × 105	1.25 × 104	0
140c	Yes	5.00 × 105	1.25 × 104	0
327c	Yes	3.125 × 106	5.25 × 107	0
349c	Yes	1.56 × 107	1.10 × 105	0
405c	Yes	1.25 × 105	2.15 × 107	0
426c	No	ND	ND	ND
431c	Yes	1.25 × 105	0	0
459c	Yes	6.25 × 105	3.25 × 106	0
590c	Yes	1.25 × 105	1.25 × 108	0
706c	Yes	2.50 × 103	3.10 × 102	0
756c	No	ND	ND	ND
823c	Yes	2.50 × 104	1.50 × 103	0
823cD6	Yes	1.50 × 107	1.50 × 105	0
885c	No	ND	ND	ND
939c	No	ND	ND	ND

^aSupernatants were collected 2 days after plasmid transfection in 293T cells or at 9 days postinoculation of the stocks into human or rhesus PBMC. ND, not done.