FIG 3.

The MCL1 transgene promotes antigen-specific CD8⁺ T cell skewing to an MPEC phenotype during primary viral infection. Mice were infected with VV-WR, and spleens and mediastinal lymph nodes were harvested on day 10 postinfection. (A) By flow cytometry, B8R⁺ CD8⁺ T cells from the spleen were assayed for expression of KLRG1 and CD127 (filled histogram, nontransgenic [Non TR]; open histogram, transgenic [TR]). The values shown are percentages of KLRG1⁺ cells or mean fluorescence intensity for CD127 in the B8R⁺ CD8⁺ gate. (B) B8R⁺ CD8⁺ T cells from mediastinal lymph node cells were assayed for expression of CD127 and KLRG1. The data shown are representative of two independent experiments with four mice per experiment. *, P < 0.05; **, P < 0.001 (for the difference between transgenic and nontransgenic animals; Student's t test).