FIG 6.

The MCL1 transgene increases the frequency and total number of memory B8R-specific CD8⁺ T cells at late times following infection. Mice were infected with VV-WR, and their spleens were harvested at day 69 postinfection. (A) Total splenocytes and CD8⁺ T cell numbers were determined. (B) Splenocytes were stained with B8R tetramer and anti-CD8 antibody and analyzed by flow cytometry. The data shown are representative of two independent experiments with six to eight mice per experiment. **, P < 0.001 (for the difference between transgenic and nontransgenic animals; Student's t test).