FIG 3.

Expression of PVX P25 by a PPV vector increases viral symptoms in Nicotiana benthamiana. (A) Representative disease symptoms induced in N. benthamiana plants by infection with PPV-P25 or PPV-GFP recombinant viruses at 15 days postagroinoculation (dpa). (B) Detached, upper leaves of PPV-P25- and PPV-GFP-infected plants were stained with DAB solution at 15 dpa (upper panels). Representative phenotypes were photographed with a Leica L2 stereoscope (lower panels). Scale bars, 0.5 mm. (C) Western blot analysis of extracts derived from upper leaves of plants infected with PPV-P25 or PPV-GFP at 15 dpa, using antibodies against the HA epitope (left panel) or PPV CP (right panel). The lower panels show the Ponceau S-stained membrane after blotting, as controls of loading.