TABLE 4.
HyperHRM analysis of editing of M-MLV, AKV, and glycosylation mutants by endogenous mA3 expressed in C57BL/6 splenocytesa
| Infected cell and virus | No. of clones analyzed | No. of mutated clones | % clones mutated | Total no. of mutations | Predicted G-to-A mutation frequency (no. of mutations/kb) |
|---|---|---|---|---|---|
| WT splenocytes | |||||
| M-MLV | 81 | 12 | 15 | 38 | 0.73 |
| M-MLV (N113Q/N505Q) | 53 | 15 | 28 | 59 | 1.72 |
| AKV | 40 | 19 | 48 | 52 | 2.00 |
| AKV (S507N) | 63 | 8 | 13 | 21 | 0.51 |
| NIH 3T3 cells | |||||
| M-MLV | 42 | 1 | 3 | 4 | 0.15 |
| M-MLV (N113Q/N505Q) | 60 | 6 | 10 | 16 | 0.41 |
| AKV | 39 | 10 | 26 | 30 | 1.19 |
| AKV (S507N) | 63 | 5 | 8 | 21 | 0.51 |
a
HyperHRM analysis was performed on gDNA from infected WT splenocytes 96 h after infection. NIH 3T3 cells were infected with viruses released from WT splenocytes. HyperHRM analysis was performed 48 h later.