FIG 3.

Limited replication of HIV-2 in human thymocytes. Viral production at the single-cell level was determined by flow cytometry using the anti-Gag antibody (Ab) KC57 at 10 days postinfection of TOCs, total thymocytes, or CD4SP thymocytes with R5- or X4-tropic HIV-1 and HIV-2 primary isolates. Representative dot plots of intracellular Gag expression (numbers show the proportion of cells inside the gate) (A) and frequency of productively infected KC57-positive thymocytes (B) under each condition are shown. Flow cytometric analysis was performed after exclusion of dead cells and aggregates. Each dot represents a single thymus. Lines indicate median values. *, P < 0.05; **, P < 0.01. FSC, forward scatter.