FIG 1.

EBV type 2 induces T-cell proliferation in cultured peripheral blood mononuclear cells. PBMCs were labeled with e450 proliferation dye and infected with EBV-1 (B95.8- or Akata-derived virus) or EBV-2 (Jijoye- or BL5-derived virus) or not infected (medium). At 7 days postinfection, cells were harvested and stained for flow cytometry. Lymphoblasts and lymphocytes were gated initially based on FSC/SSC, followed by CD19⁺ B and CD3⁺ T cells, and subsequently, proliferated cells were determined by e450 dilution. (A) Representative histograms of CD19⁺ B-cell and CD3⁺ T-cell proliferation measured by fluorescence intensity of the e450 proliferation dye from 3 to 9 replicates of 4 independent experiments. (B) Mean frequencies of B- and T-cell proliferation. The error bars represent means ± standard errors. ***, P < 0.001, and ns, P > 0.05, using a two-way ANOVA with Bonferroni's posttest.