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. 2014 Nov 26;89(4):2052–2063. doi: 10.1128/JVI.01106-14

FIG 5.

FIG 5

Relative motions in the thumb domain and C-terminal arm of the Y448F and W550N mutants. (A) The superposition of the W550N mutant and WT NS5B (top) and the corresponding deviations in α-carbon positions (bottom) show that the mutant structure adopts a more open conformation. This is particularly evident for the thumb domain (residues 370 to 528) and even more so for the C-terminal arm (residues 528 to 566). The large deviations around positions 25 and 150 correspond to the flexible fingertips, long extensions within the NS5B finger domain. (B) Comparison of the Y448F mutant and WT NS5B shows smaller structural differences, with consistently around or less than a 0.5-Å difference in α-carbon positions, providing some structural underpinning toward the more wild-type-like phenotype of the Y448F mutant.