FIG 6.

Sedimentation velocity analytical ultracentrifugation analysis of WT NS5B and the W550A and W550N C-terminal arm mutants. Experiments were performed with 2 μM samples in a solution containing 10 mM Tris (pH 7.5), 600 mM NaCl, 10% glycerol, and 5 mM β-mercaptoethanol at 7°C.