FIG 2.

gM drives gN to the TGN in transfected cells. 143B cells grown on coverslips were transfected with plasmids coding for HA-tagged gM and YFP-labeled gN or cotransfected with the two plasmids and processed as described in Materials and Methods. The coverslips were fixed and then reacted with antibodies against HA (to detect gM), TGN46 (to label the TGN), or calnexin (to identify the endoplasmic reticulum), while nuclei were labeled with Hoechst. Samples were analyzed by confocal laser scanning microscopy. Scale bar, 10 μm.