FIG 3.

Physical interaction between gM and gN in transfected cells. HeLa cells were transfected with plasmids expressing HA-tagged gM (gM) or YFP-tagged gN (gN) or cotransfected with both constructs (gMgN). The cells were harvested at 24 h posttransfection and lysed with RIPA buffer. (A) As positive controls, 1/50th of the total lysate of each sample was also loaded onto the gels and analyzed. (B and C) The rest of the lysates were immunoprecipitated (IP) with mouse anti-HA antibodies for gM or mouse anti-GFP antibodies for gN and subjected to SDS-PAGE in 10% gel followed by Western blotting. The blots were reacted with the PAS980 anti-gM antibody or an anti-GFP antibody to detect gM and gN, respectively, as indicated to the left of each blot. The molecular masses of the molecular weight markers are indicated to the right of the panels.