FIG 4.

Coimmunoprecipitation of the gM/gN complex in infected cells. HeLa cells were transfected with YFP-tagged gN for 24 h. They were then infected with wild-type virus at an MOI of 5, and the cells were harvested at 16 hpi and lysed. Two and a half percent (1/40th) of the lysates were directly loaded onto the gels (A), and the rest of the lysates were immunoprecipitated (IP) with the 4C10 anti-gM or anti-GFP antibody as indicated (B and C). All samples were analyzed by Western blotting. The blots were reacted with the PAS980 anti-gM (A to C, right side) or anti-GFP antibodies (A to C, left side). Uninfected cells were used as the negative control. The molecular masses of the molecular weight markers are indicated to the right of the panels.