FIG 6.

gM/gN interacts less efficiently with the amino-truncated ΔgM2 protein. HeLa cells were transfected with YFP-tagged gN for 24 h. They were then infected with HSV-1ΔgM2 virus at an MOI of 5, and the cells were harvested at 16 hpi and lysed. Two and a half percent (1/40th) of the lysates were directly loaded onto the gels, and the rest of the lysates were immunoprecipitated (IP) with the 4C10 anti-gM or anti-GFP antibody (B and C) and analyzed by Western blotting. The blots were reacted with the PAS980 anti-gM (A to C, right side) or anti-GFP antibodies (A to C, left side). As before, the molecular masses of the molecular weight marker are indicated to the right of the panels.