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. 2014 Dec 3;89(4):1975–1985. doi: 10.1128/JVI.03253-14

FIG 6.

FIG 6

M2 proton channel function during HA biosynthesis accounts for the enhancement of HA-mediated cell-cell fusion. (A) Amantadine (AM) treatment windows. Treat 1, amantadine was maintained from 0.5 h post-HA and -M2 transfection of effector cells and thereafter, until the fusion assay ended, at the point of lysing cells; treat 2, amantadine was maintained from the time of low-pH treatment of effector-target cocultures and thereafter, until the fusion assay ended, at the point of lysing cells; treat 3, amantadine was maintained from the point right after the pH treatment of effector-target cocultures and thereafter, until the fusion assay ended, at the point of lysing cells. (B) Effects of amantadine treatment on MX M2-N31S enhancement of MX HA-mediated cell-cell fusion at pH 5.0. Amantadine (10 μM final concentration) was maintained according to treatments 1, 2, and 3. Fusion data are shown as means and standard deviations for three independent experiments. Plasmids used for transfection included 1 μg of MX HA plasmid and 3 μg of MX M2-N31S plasmid. *, P < 0.001 (compared to no AM treatment; t test).