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Nitric oxide synthase 3 (NOS3) expression in thick ascending limb suspensions obtained from ANG II- and vehicle-infused rats after 5 days of treatment. Paired vehicle and ANG II samples were loaded in the same gel. NOS3 expression levels were corrected by β-tubulin and values were expressed as percentage of vehicle. ANG II infusion for 5 days decreased NOS3 expression by 40 ± 12% (P < 0.007; n = 6).
