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. 2014 Nov 5;308(2):F149–F156. doi: 10.1152/ajprenal.00279.2014

Fig. 6.

Fig. 6.

Effect of PIP3 stimulation on NOS3 phosphorylation at S1177. Thick ascending limb suspensions obtained from ANG II- and vehicle-infused rats after 5 days of treatment were split, incubated at 37°C for 10 min, and then treated with either PIP3 plus carrier or carrier alone for 8 min. Phospho (P)-NOS3 (S1177) levels were corrected by NOS3 expression and results were expressed as percentage of P-NOS3 (S1177) in thick ascending limbs treated with carrier. A: PIP3 stimulation increased P-NOS3 (S1177) in thick ascending limbs from vehicle-infused animals by 18 ± 4% (P < 0.02; n = 5). B: PIP3 stimulation had no effect on P-NOS3 (S1177) in thick ascending limbs from ANG II-infused animals compared with carrier (ns; n = 5).