Table 1.

Antibodies and blotting conditions

					Conditions
Antibody	Provider	Catalog Number	Source	Blocking Buffer	Dilution	Buffer	Time	Temp.
NOS3	BD Bioscience	610296	Mouse	5% Milk†	1:1,000	5% Milk	1 h	Room T.
pNOS3 (S1177)	BD Bioscience	612392	Mouse	5% Milk	1:2,000	5% Milk	1 h	Room T.
pNOS3 (T495)	CellSignaling	9574	Rabbit	BlokTM-PO*	1:500	BlokTM-PO	15 h	4°C
ETB receptor	Alomone	AER-002	Rabbit	5% Milk	1:1,250	5% Milk	1 h	Room T.
β-Tubulin	Abcam	ab6046	Rabbit	5% Milk	1:10,000	5% Milk	1 h	Room T.
2ry anti-rabbit-HRP	GE Healthcare	NA9340V	Donkey		1:2,500	5% Milk	1 h	Room T.
2ry anti-mice-HRP	GE Healthcare	NA931V	Sheep		1:1,000	5% Milk	1 h	Room T.

^*Blok TM-PO (Millipore, cat. no.: WBAVDP001).

^†5% Milk: 50 g/l nonfat milk dissolved in 20 mM Tris (pH 7.6), 137 mM NaCl, and 0.1% Tween 20.