Fig. 7.

Apoptotic cell death induced by different concentrations of Baf-A₁ (Baf). Representative FACS histograms for A549 cells treated with different concentrations of Baf-A₁ (24 h) (indicated by text in relevant panels), and infected with influenza A/PR/8/34 virus (MOI 1 PFU/cell). For controls, cultures either underwent Mock infection or were not treated at all (labeled as Control). Cells were lifted, and nuclei were stained with propidium iodide, then subjected to FACS analysis to identify apoptotic cells with a reduced DNA content, called the sub-G₁ subgroup (delineated as M1). B: bar graph showing mean data for fraction of cells in sub-G1 subgroup) from FACS analyses displayed in A. White bar represents the Control condition, whereas dark gray bar (2nd from left) is for mock-infected cultures. Results represent means ± SD from 6 independent experiments. ***P < 0.001 compared with Control. *P < 0.01 compared with Mock; **P < 0.001 compared with Mock.