Fig. 5.
Cell retention in mouse lungs. 0.5 × 106 GFP-labeled NHBE cells were delivered to mouse lungs, and the retention of delivered cells was determined after 2 days. A–C: lung sections from control mice with no cell delivery. D–F: lung sections from animals with cell delivery only. G–I: lung sections from animals received cell delivery 24 h following pretreatment with 2% PDOC. Lung sections were immunostained with rabbit polyclonal antibody to GFP followed by Alexa Fluor 555-conjugated IgG (A, D, and G) and counterstained with DAPI (B, E, and H) or stained for H&E (C, F, and I). J: cell-retention efficiency was determined by GFP ELISA. CELLS, cell delivery only; PDOC+CELLS, cell delivery following lung pretreatment with PDOC. *P < 0.05.