Fig. 6.
Retention of cells in pig lungs. 100 × 106 GFP-labeled A549 cells were delivered to 1 lobe of the pig lung, and the cell retention was determined after 24 h. A–C: lung sections from control pigs. D–F: lung sections from animals received cell delivery. Lung sections were immunostained with rabbit polyclonal antibody to GFP followed by Alexa Fluor 555-conjugated IgG (A and D) and counterstained with DAPI (B and E) or stained for H&E (C and F). G: number of engrafted cells in random sections of the pig lung of various weights.