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. 2014 Nov 21;308(2):L221–L228. doi: 10.1152/ajplung.00147.2014

Fig. 7.

Fig. 7.

Expression and localization of CFTR-GFP in NHBE cells. A: flow cytometry analysis of NHBE cells infected by pSicoR-CFTR-GFP lentivirus. BE: confocal images of NHBE cells infected with pSicoR-CFTR-GFP lentivirus. B: GFP fluorescence. C: CFTR immunofluorescence. CFTR was detected with mouse monoclonal anti-CFTR followed by Alexa Fluor 555-conjugated IgG (red). D and E: overlay of GFP fluorescence, CFTR immunofluorescence, and DAPI staining. Arrows, overexpression of CFTR-GFP.