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. 2015 Jan 29;4:1–7. doi: 10.1016/j.gdata.2015.01.008
Specifications
Organism/cell line Mus musculus cell lines G1E and G1E-ER4
Strain 129
Sex Male
Sequencer or array type Illumina Genome Analyzer IIx, Illumina HiSeq 2000
Data format Sequence reads: fastq; mapped reads: bam, bai (bam index file); peaks calls: broadPeak; signal tracks: bigwig
Experimental factors Mouse cell line (G1E) with a genetic knockout of the Gata1 gene and a daughter cell line (G1E-ER4) transduced with an estrogen-activated Gata1-estrogen receptor transgene that can rescue erythroid maturation in an estrogen-dependent manner.
Experimental features G1E cells (untreated) and G1E-ER4 cells treated with 10 nM estradiol for six time points (0, 3, 7, 14, 24, and 30 h) were (a) analyzed genome-wide for binding by GATA1 using ChIP-seq (including control samples of input DNA at each time point) and (b) mapped for transcription genome-wide by strand-specific, paired-end RNA-seq of poly A + RNA, including biological replicates.
Consent Not applicable
Sample source location Not applicable