Organism/cell line |
Mus musculus cell lines G1E and G1E-ER4 |
Strain |
129 |
Sex |
Male |
Sequencer or array type |
Illumina Genome Analyzer IIx, Illumina HiSeq 2000 |
Data format |
Sequence reads: fastq; mapped reads: bam, bai (bam index file); peaks calls: broadPeak; signal tracks: bigwig |
Experimental factors |
Mouse cell line (G1E) with a genetic knockout of the Gata1 gene and a daughter cell line (G1E-ER4) transduced with an estrogen-activated Gata1-estrogen receptor transgene that can rescue erythroid maturation in an estrogen-dependent manner. |
Experimental features |
G1E cells (untreated) and G1E-ER4 cells treated with 10 nM estradiol for six time points (0, 3, 7, 14, 24, and 30 h) were (a) analyzed genome-wide for binding by GATA1 using ChIP-seq (including control samples of input DNA at each time point) and (b) mapped for transcription genome-wide by strand-specific, paired-end RNA-seq of poly A + RNA, including biological replicates. |
Consent |
Not applicable |
Sample source location |
Not applicable |