Table 2.
The means of CI 50 of the SYBR green based in vitro anti-malarial drugs assay according to protocols
| Clone/drugs | Protocol standard | New Protocol (test) | P | ||
|---|---|---|---|---|---|
| Mean CI 50 (nM) | 95% CI | Mean CI 50 (nM) | 95% CI | ||
| 3D7 | |||||
| CQ | 31.10 ± 6.02 | 24.28-37.91 | 32.43 ± 4.31 | 26.22-35.97 | 0.77 |
| DHA | 2.30 ± 0.68 | 1.53-3.06 | 1.93 ± 0.43 | 1.81-2.78 | 0.47 |
| PYD | 8.82 ± 1.17 | 7.49-10.14 | 8.89 ± 1.54 | 7.07-10.56 | 0.97 |
| PPQ | 26.33 ± 1.71 | 21.38-28.27 | 25.66 ± 6.82 | 15.61-34.4 | 0.88 |
| W2 | |||||
| CQ | 467.66 ± 51.18 | 409.74-525.57 | 500.66 ± 87.22 | 368.96-566.35 | 0.60 |
| DHA | 1.94 ± 1.03 | 0.77-3.10 | 1.81 ± 0.99 | 0.81-3.06 | 0.81 |
| PYD | 5.35 ± 1.27 | 3.90-6.79 | 4.33 ± 1.21 | 3.98-6.71 | 0.37 |
| PPQ | 19.50 ± 2.91 | 16.20-22.79 | 17.20 ± 3.14 | 15.94-23.05 | 0.40 |
| HB3 | |||||
| CQ | 37.92 ± 6.66 | 30.38-45.47 | 50.28 ± 16.77 | 31.30-69.26 | 0.33 |
| DHA | 2.10 ± 0.97 | 0.99-3.20 | 1.99 ± 0.41 | 1.53-2.46 | 0.87 |
| PYD | 11.78 ± 2.36 | 9.10-14.46 | 12.51 ± 3.40 | 8.65-16.36 | 0.70 |
| PPQ | 21.70 ± 1.76 | 19.70-23.70 | 18.51 ± 2.24 | 15.97-21.06 | 0.12 |
| 7G8 | |||||
| CQ | 416.98 ± 92.57 | 311.82-521.33 | 420.55 ± 96.04 | 311.87-529.24 | 0.18 |
| DHA | 0.65 ± 0.17 | 0.45-0.85 | 0.73 ± 0.12 | 0.58-0.87 | 0.56 |
| PYD | 4.43 ± 1.39 | 2.84-6.01 | 5.05 ± 0.34 | 4.66-5.44 | 0.52 |
| PPQ | 17.82 ± 3.57 | 13.77-21.87 | 15.98 ± 1.22 | 14.59-17.38 | 0.47 |
CQ = Chloroquine; DHA = Dihydroartemisinin; PPQ = Piperaquine; PYD = Pyronaridine; SD = Standard deviation, nM = nanomolar.