Table 1.
Glucose uptake and clearance rate measurements with FLIPglu-600µM in cell lines
| Uptake rates | Clearance rates | |||||||
|---|---|---|---|---|---|---|---|---|
| Physiological glucose | High glucose | Physiological glucose | High glucose | |||||
| Cell type | τd1 | τd2 | τd1 | τd2 | τr1 | τr2 | τr1 | τr2 |
| HEK 293 | 29±7 (n=9) | 38±15 (n=11) | 42±9 (n=9) | 21±10 (n=11) | ||||
| CHO | 16.3±5 (n=11) | 49±13 (n=10) | 26±11 (n=11) | 28.5±12 (n=10) | ||||
| COS-7 | 12±2.5 (n=6) | 160 (n=2) | 16±2.5 (n=6) | 10±1.5 (n=6) | 150(n=2) | 13.5±5 (n=6) | ||
| C2C12 | 9±3 (n=18) | 25±6.5 |(n=8) | 13±7.5 (n=14) | 102±54 (n=12) | 175±7 (n=18) | 22.5±6 (n=14) | 320±150 (n=7) | |
Time constant values in seconds obtained from fitting the decrease and increase in FRET ratio observed upon addition and removal of 10 mM glucose to the bath solution. In most cases, a good fit could be achieved with a single exponential; however, when this was not satisfactory, a second component was introduced. The very slow component observed after prolonged exposure to IAA (τ =1,000 to 2,000 s) has not been included in this table and is reported in the appropriate result section.