A. Two or more KLFs may both be regulatory targets of the same NR and activate or repress the same downstream gene. However, there is no additive or synergistic effect of the multiple KLFs, and any one protein can function. B. Two KLFs in the same pathway may have synergistic effects, perhaps by binding to separate response elements at the same locus (direct synergy) or by regulating other genes that contribute to the transcriptional effect (e.g. repressing the expression of a repressor). C. Two or more KLFs may have opposing or antagonistic actions. In the diagrammed example, a KLF that is upregulated early in the NR response activates a target gene while a different KLF that is upregulated later represses it, allowing a temporally restricted response to the NR.