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Process monitoring of cell cultures over batch subculture period. a Aggregate distribution data obtained on a Coulter counter over successive days of the batch cycle, each curve represents the average of two 2mL samples. b Growth curve obtained from distribution data; biomass correlation (Fig. 3c) used to calculate dry weight from Coulter counter data, substrate concentration is the sum of sucrose and glucose in the culture medium. c Mean diameter of aggregates over batch cycle
