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. Author manuscript; available in PMC: 2016 Feb 2.
Published in final edited form as: Curr Protoc Protein Sci. 2015 Feb 2;79:30.4.1–30.4.30. doi: 10.1002/0471140864.ps3004s79

Figure 1.

Figure 1

Cloning strategy to generate the iPhage library. The f88-4 phage vector contains two capsid genes encoding a wild-type (wt) protein VIII (pVIII) and a recombinant protein VIII (rpVIII). The recombinant gene VIII contains a foreign DNA insert with a HindIII and a PstI cloning site. The tac promoter controls the expression of the rpVIII. Annealed oligonucleotides encoding the penetratin (pen) peptide are cloned in frame with the rpVIII (f88-4). Next, the fUSE5 and f88-4/pen genomes are fused to produce the iPhage display vector. The PCR-insert library is cloned into the SfiI endonuclease site. Representation of the assembled phage particle expressing the wt major coat protein pVIII (gray), rpVIII-pen (green); rpIII, minor coat protein (red-square); TetR, tetracycline resistance gene (white).