Component	Amount per reaction	Final
X4YX4 Template	0.1 µl	100 ng
Library Forward primer	3.0 µl	3 µg
Library Reverse primer	3.0 µl	3 µg
DMSO	1.0 µl	2%
10 mM dNTPs	2.0 µl	0.4 mM
25 mM MgCl2	2.4 µl	1.2 mM
5× GoTaq buffer	10.0 µl	1×
GoTaq polymerase (5U/µl)	1.0 µl	5U
Milli-Q water	Up to 50 µl