| Component | Amount per reaction | Final |
|---|---|---|
| Vector | Depends on concentration | 50 ng |
| Insert | Depends on concentration | e.g. 1.02 ng |
| 5× Ligation buffer | 4.0 µl | 1× |
| T4 DNA ligase (5 U/µl) | 1.0 µl | 5 U |
| Milli-Q water | Up to 20.0 µl |
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