Component	Amount per reaction	Final
Bacterial suspension	2.0 µl
pIIIseq forward primer	1.0 µl	8 pmol
pIIIseq reverse primer	1.0 µl	8 pmol
10 mM dNTP	0.5 µl	0.25 mM
5× GoTaq buffer	4.0 µl	1×
DMSO	0.4 µl	2%
GoTaq polymerase (5U/µl)	0.4 µl	2U
Milli-Q water	Up to 20 µl