| Step | Temperature (°C) | Time | Cycles |
|---|---|---|---|
| Initial Denaturation | 94 | 3 min | 1 |
| Denaturation | 94 | 10 sec | 35 |
| Annealing | 60 | 30 sec | |
| Extension | 72 | 1 min | |
| Final extension | 72 | 3 min | 1 |
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