Fig. 2.

Leukocyte infiltration and demyelination in CPN^−/− mice during EAE. Spinal cords from wild type and CPN^−/− mice (n=4 for each group) were obtained at 30 days post-immunization, fixed in 10% buffered formalin and paraffin-embedded. Sections from the cervical, thoracic and lumbar regions (5μm) were stained with H&E (A and C) or Luxol fast blue (B and D) and scored as described in the Materials and Methods. (A, B) In representative sections from a CPN^−/− mouse, infiltration is largely limited to the meninges and the ventral fissure (arrows) and there is little inflammation or demyelination. (C,D) In representative sections from a wild type mouse stained there is extensive parenchymal and meningeal infiltration combined with inflammation and a moth-eaten appearance and extensive demyelination and inflammation compared to CPN^−/− mice (arrows). Original magnification 20X.